insulin SKIP PCR, insert, cloning & identify steps. Brief summary: has no audio, skips amplify & identify.
restriction SAY complementary=sticky, Random! Palindrome MADAM 01/02/2010, 11/02/2011. STEP ON NO PETS. Gene Editing Using CRISPR: Clustered Regularly Interspaced Short Palindromic Repeats (Cleveland Clinic) Genome Editing with CRISPR-Cas9 (McGovern Institute for Brain Research at MIT)
GMO Genetic bacterial engineering could herald new era of biofuels Ebola vaccine in GM tobacco plants GMO Soybeans & Sustainability: herbicide and no-till Genetically Modified Salmon Coming Soon? (Link TV) Genetically modified mosquitoes bred to fight Zika BRING non GMO bread. CORN is unnatural! Also BANANA unnatural! Draught tolerant almond?
Corn tassel=male flower, silk=female; detassel to cross-pollinate.
Pork insulin 1 amino acid diff. Knockout mouse: inactivated gene.
Bt European corn borer; Bt crystal: pore in gut cell membrane. 5.14 MONARCH cats?
Glyphosate inhibits aromatic a.a. (contains a ring - phenylalanine) - active growth
golden Rice self-pollinated (vitamin A night blindness) FDA: Generally Recognized as Safe; substantially equivalent. Golden rice: enriched with beta carotene to treat vitamin A deficiency (PRI) The Future of Food 25:30-30:00 engineering Roundup Ready corn
cloned BANANA seedless fruit clone underground stem: sucker, cutting. PEANUT embryo
White Dorset, Scottish Blackface egg. Explain SURROGATE.
The 4 HHMI is about embryonic stem cells. Therapeutic cloning I : nuclear transfer (HHMI) Therapeutic cloning II : making a blastocyst (HHMI) Therapeutic cloning III : blastocyst development (HHMI) Therapeutic cloning IV : growing stem cells with Sonic Hedgehog (HHMI) First baby born with DNA from 3 parents First baby born with 3 parent technique: nuclear transfer 87MB NOVA-scienceNOW 3:30 IVF clinics, 5:30-10:30 11:40-13:40 remove IPS oncogene
STR Define TANDEM can be end-to-end like palindrome. 10 different alleles per STR, 13 different STR locations.
electrophoresis Agar-agar ANODE, polyacrylamide semi-solid w pores 1987 Colin Pitchfork RFLP as a genetic diagnostic tool: determining a person's genotype by gel electrophoresis
fingerprint NOVA-scienceNOW 2:20 GATTACA 4:30 SNP - 10:30 PSA test
76 MB Neil deGrasse Tyson; amniocentesis. Colin Pitchfork: the gel is original INNOCENT suspect. Preimplantation Genetic Diagnosis (PGD) Breeding out disease (CBS 60 Minutes, 10/26, 2014)1:30 - 6:45 Personal DNA testing 4:30 - 10:30 What are your genetic risk factors? (NOVA scienceNOW)
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