Letter "e" under low power and high power.

1. How does the position or movement of an object change when viewed under the compound microscope?
   The position is reversed.

2. If an organism swims to the left on the slide, which way will it swim as you view it through the microscope?
   To the right.

   If it swims down, which way will it appear to swim?
   Up.

3. When you switch from low to high power, explain what changes occur for each of the following for the letter "e":
   1. The position of the specimen:
      should not change - you should see just a smaller portion of the letter.
   2. The size of the field of view:
      smaller field of view.
   3. The amount of available light:
      less light is available - letter appears dimmer.
   4. The proportion of the specimen that can be seen:
      less of the specimen is visible.

Colored Threads: High Power (400X)

Hydra: High Power (400X)

Low (100x) 1.5 mm = 1,500 µm
High 400x) 0.4 mm = 400 µm

Width of Letter "e" LP: 0.9 mm = 900 µm
Width of Hydra Body Stalk LP: 0.10 mm = 100 µm
Width of Hydra Tentacle LP: 0.02 mm = 20 µm

Problem:
The diagram below allows you to determine the diameter of the field of view of a microscope at 100X.

Diameter at 100X = 1.6 mm or 1,600 µm

Diameter at 400x = 0.4 mm or 400 µm

If 4 amoebas fit across the field of view of the same microscope at 400X, what is the approximate size of one amoeba?

Field of view at low power is about 1.6 mm.
Field of view at high power is about 1.6 mm / 4 = 0.4 mm.
Size of one amoeba: 0.4 mm / 4 = 0.1 mm = 100 µm.