Introduction.

• PART A - Microscope Care and Safety Rules

• PART B - Parts of the Microscope and Their Functions p. 42

  Figure 5-1 The compound microscope

  Leica DM500 Please hold the microscope with 2 hands: by the arm and base.

• PART C - Making a Wet Mount Slide p. 43 Use a small lower case "e" for first specimen.

  You may choose to take photos to supplement your sketches on the data sheet.

• PART D - Focusing the Microscope p. 44

  Start with scanning power, then try observing the letter under low power and high power.

• PART E - Depth of Field p. 45

  Observe depth of field on 3-dimensional objects: prepared slides of colored threads (cross fibers).

  Which color is the top, middle, and bottom?

• PART F - Measuring the Field of View

  Figure 5-3 Measuring the field of view on low power (100X)

  Estimate width of field by comparing with mm marks on the ruler.

  Note: do not try to measure field of view under high power (400X).
  Just divide the low power value by 4; see item 4 on p. 46.

  Estimate size of specimen: diameter of field of view / # of times specimen fits across: estimate size of letter "e" and hydra.

Optional - DISSECTING MICROSCOPE

Leica Zoom 2000