Restriction enzymes can be used to isolate genes of interest.
- A gene of interest from a target organism is identified.
- A restriction enzyme can cut DNA
double helixes at specific restriction sites that contain a palindrome sequence.
- The restriction enzyme binds and cuts DNA
at the restriction sites.
- DNA fragments with complementary single-stranded ends are produced.
Some of these fragments contain the genes of interest.
After isolation, restriction fragments can reform double helixes with other sources of DNA
to produce recombinant DNA.
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