1. RNA polymerase binds to DNA at a specific region called the promoter site and separates the double helix to single strands.

  2. One of the DNA strands on the gene is used as a template on which RNA nucleotides are built, using the base-pairing rules. Note:

  3. The mRNA transcript is released when it reaches the end of the gene.

  4. The intron portions of the transcript are removed.